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KMID : 1033620110380030135
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Clinical and Experimental Reproductive Medicine
2011 Volume.38 No. 3 p.135 ~ p.141
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Expression of SDF-1α and leptin, and their effect on expression of angiogenic factors in mouse ovaries
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Park Min-Jung
Park Sea-Hee
Lee Su-Kyung
Joo Bo-Sun
Moon Sung-Eun
Moon Hwa-Sook
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Abstract
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Objective:Ovarian angiogenesis plays an important role in folliculogenesis. However, little is known about the expression of angiogenic factors during follicular development according to female age. Stromal cell derived factor-1α (SDF-1α) plays a role in granulosa cell survival and embryo quality as an angiogenic chemokine. Leptin is also involved in folliculogenesis and angiogenesis. This study examined expression of SDF-1α and leptin, and their effects on the expression of angiogenic factors in the ovary during follicular development according to female age.
Methods:Ovaries were collected from C57BL mice of two age groups (6-9 weeks and 24-26 weeks) at 6, 12, 24, and 48 hours after 5 IU pregnant mare’s serum gonadotropin (PMSG) injection. The expression of ovarian SDF-1α and leptin mRNA was evaluated by RT-PCR. In the organ culture experiment, the ovaries were cultured in transwell permeable supports with Waymouth’s medium treated with various doses of SDF-1α (50-200 ng/mL) or leptin (0.01-1 μg/mL) for 7 days. Then, mRNA expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), and visfatin were examined in the cultured ovaries.
Results:Expression of SDF-1α and leptin in the ovary was significantly lower in the aged mouse group compared to the young mouse group (p<0.05). Expression of these two factors increased with follicular development after PMSG administration. SDF-1α treatment stimulated visfatin expression in a dose-dependent manner, while leptin treatment significantly increased eNOS expression.
Conclusion:These results suggest that decrease of ovarian SDF-1α and leptin expression may be associated with aging-related reduction of ovarian function. SDF-1α and leptin may play a role in follicular development by regulating the expression of angiogenic factors in mouse ovaries.
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KEYWORD
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Ovary, Angiogenesis, Aging, Stromal Cell Derived Factor-1 Alpha, Leptin, Mouse
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